The rapid spread of epidemic diseases (i.e., coronavirus disease 2019 (COVID-19)) has contributed to focus global attention on the diagnosis of medical conditions by ultrasensitive detection methods. To overcome this challenge, increasing efforts have been driven towards the development of single-molecule analytical platforms. In this context, recent progress in plasmonic biosensing has enabled the design of novel detection strategies capable of targeting individual molecules while evaluating their binding affinity and biological interactions. This review compiles the latest advances in plasmonic technologies for monitoring clinically relevant biomarkers at the single-molecule level. Functional applications are discussed according to plasmonic sensing modes based on either nanoapertures or nanoparticle approaches. A special focus was devoted to new analytical developments involving a wide variety of analytes (e.g., proteins, living cells, nucleic acids and viruses). The utility of plasmonic-based single-molecule analysis for personalized medicine, considering technological limitations and future prospects, is also overviewed.
【저자키워드】 virus, biosensors, nucleic acids, plasmonics, nanoparticle, nanostructure, single-molecule analysis, living-cells, 【초록키워드】 coronavirus disease, viruses, Biomarker, Diagnosis, Proteins, binding affinity, Spread, nucleic acid, Personalized medicine, utility, interactions, Analysis, epidemic disease, approaches, effort, limitation, living cells, clinically, variety, overcome, contributed, medical condition, 【제목키워드】 biosensor,