Objectives The purpose of the current study was to evaluate the analytical performance of seven kits for detecting IgM/IgG antibodies against coronavirus (SARS-CoV-2) by using four chemiluminescence immunoassay systems. Methods Fifty patients diagnosed with SARS-CoV-2 infection and 130 controls without coronavirus infection from the General Hospital of Chongqing were enrolled in the current retrospective study. Four chemiluminescence immunoassay systems, including seven IgM/IgG antibody detection kits for SARS-CoV-2 (A_IgM, A_IgG, B_IgM, B_IgG, C_IgM, C_IgG and D_Ab), were employed to detect antibody concentrations. The chi-square test, the receiver operating characteristic (ROC) curve and Youden’s index were determined to verify the cut-off value of each detection system. Results The repeatability verification results of the A, B, C and D systems are all qualified. D_Ab performed best (92% sensitivity and 99.23% specificity), and B_IgM performed worse than the other systems. Except for the A_IgM and C_IgG systems, the optimal diagnostic thresholds and cut-off values of the other kits and their recommendations are inconsistent with each other. B_IgM had the worst AUC, and C_IgG had the best diagnostic accuracy. More importantly, the B_IgG system had the highest false-positive rate for testing patients with AIDS, tumours and pregnancies. The A_IgM system test showed the highest false-positive rates among elderly individuals over 90 years old. COVID-2019 IgM/IgG antibody test systems exhibit performance differences. Conclusions The Innodx Biotech Total Antibody serum diagnosis kit is the most reliable detection system for anti-SARS-CoV-2 antibodies, which can be used together with nucleic acid tests as an alternative method for SARS-CoV-2 detecting.
【저자키워드】 COVID-19, SARS-CoV-2, antibody, chemiluminescence immunoassay, performance verification, 【초록키워드】 Coronavirus infection, coronavirus, SARS-COV-2 infection, diagnostic, Diagnosis, COVID-2019, Retrospective study, serum, sensitivity, specificity, anti-SARS-CoV-2 antibodies, Antibody detection, ROC, Accuracy, IgM/IgG, AIDS, nucleic acid test, Patient, Control, Antibody test, threshold, characteristic, False-positive, AUC, chi-square test, individual, Antibody concentrations, cut-off value, pregnancies, recommendation, objective, Chongqing, detection system, Seven, Result, enrolled, highest, performed, detect, evaluate, diagnosed, can be used, Total, false-positive rate, tumour, 【제목키워드】 Antibody detection, performance,