Background A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries. Methods In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV was developed and compared with conventional agarose gel-based RT-PCR for the detection of IBV infection. Results We found that the SYBR green I real-time RT-PCR was at least 10 times more sensitive than the agarose gel electrophoresis detection method. The assay exhibited high specificity for IBV infection. All negative controls, such as Newcastle disease virus, infectious bursal disease virus, and avian influenza virus, were not detected. Conclusion The SYBR green I real-time RT-PCR test described herein can be used to rapidly distinguish IBV from other respiratory pathogens, which is important for diagnosis and control of infectious bronchitis outbreaks in Morocco. The test is a valuable and useful method as a routine assay for diagnosis of clinical IBV infection in commercial chickens.
【저자키워드】 coronavirus, RT-PCR, SYBR Green, real time RT-PCR, Infectious Bronchitis Virus, Gammacoronavirus, IBV, 【초록키워드】 Infection, Diagnosis, Influenza virus, virus, specificity, outbreak, Respiratory pathogens, molecular, real-time RT-PCR, disease, newcastle disease virus, Nucleocapsid gene, Morocco, negative controls, bronchitis, IBV infection, real-time RT-PCR test, agarose gel electrophoresis, polymerase chain, real-time reverse transcriptase, Result, described, exhibited, can be used, 【제목키워드】 Infection, Diagnosis, real-time RT-PCR, Morocco,