The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis -acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5′-untranslated region (5′-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5′-UUUCGU-3′ hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive 1 H, 13 C and 15 N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy. Supplementary Information The online version contains supplementary material available at 10.1007/s12104-021-10007-w.
【저자키워드】 SARS-CoV-2, 5′-UTR, COVID19-NMR, Solution NMR spectroscopy, SL5a, 【초록키워드】 translation, COVID-19 pandemic, Genome, virus, antiviral drug, RNA, Replication, viral replication, NMR spectroscopy, targets, nucleotide, 5′-untranslated region, Major, supplementary material, sequence, RNA genome, positive, element, 5′-UTR, asymmetric, Affect, feature, single-stranded, conserved, 【제목키워드】 5′-UTR,