Abstract Motivation SARS-CoV-2 is a novel coronavirus currently causing a pandemic. Here, we performed a combined in-silico and cell culture comparison of SARS-CoV-2 and the closely related SARS-CoV. Results Many amino acid positions are differentially conserved between SARS-CoV-2 and SARS-CoV, which reflects the discrepancies in virus behaviour, i.e. more effective human-to-human transmission of SARS-CoV-2 and higher mortality associated with SARS-CoV. Variations in the S protein (mediates virus entry) were associated with differences in its interaction with ACE2 (cellular S receptor) and sensitivity to TMPRSS2 (enables virus entry via S cleavage) inhibition. Anti-ACE2 antibodies more strongly inhibited SARS-CoV than SARS-CoV-2 infection, probably due to a stronger SARS-CoV-2 S-ACE2 affinity relative to SARS-CoV S. Moreover, SARS-CoV-2 and SARS-CoV displayed differences in cell tropism. Cellular ACE2 and TMPRSS2 levels did not indicate susceptibility to SARS-CoV-2. In conclusion, we identified genomic variation between SARS-CoV-2 and SARS-CoV that may reflect the differences in their clinical and biological behaviour. Supplementary information Supplementary data are available at Bioinformatics online.
【저자키워드】 TMPRSS2, 【초록키워드】 SARS-CoV-2, ACE2, pandemic, Mortality, antibody, SARS-CoV, motivation, SARS-COV-2 infection, susceptibility, virus, Novel coronavirus, sensitivity, Cell culture, virus entry, cleavage, cell tropism, receptor, in-silico, information, Amino acid, cellular, Interaction, human-to-human transmission, genomic variation, discrepancy, SARS-CoV S, effective, TMPRSS2 level, Result, performed, conserved, inhibited, the S protein, reflect, 【제목키워드】 SARS-CoV-2, SARS-CoV, Amino acid, conserved,