Phenotypic assays are increasingly employed to provide clues about drug mechanisms. In antimalarial drug screening, however, the majority of assays are designed to only measure parasite-killing activity. We describe here a high-content assay to detect drug-mediated perturbation of the digestive vacuole integrity in the trophozoite stage of Plasmodium falciparum, using the ImageStream imaging flow cytometer.
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【저자키워드】 Apoptosis, Drug discovery, malaria, Plasmodium falciparum, programmed cell death, digestive vacuole, Fluo-4, Imaging flow cytometry,
【저자키워드】 Apoptosis, Drug discovery, malaria, Plasmodium falciparum, programmed cell death, digestive vacuole, Fluo-4, Imaging flow cytometry,