Acute lung injury (ALI) models are characterized by neutrophil accumulation, tissue damage, alteration of the alveolar capillary membrane, and physiological dysfunction. Lipoxin A_{4} (LXA_{4} ) is an anti-inflammatory eicosanoid that was demonstrated to attenuate lipopolysaccharide-induced ALI. Experimental models of severe malaria can be associated with lung injury. However, to date, a putative effect of LXA_{4} on malaria (M)-induced ALI has not been addressed. In this study, we evaluated whether LXA_{4} exerts an effect on M-ALI. Male C57BL/6 mice were randomly assigned to the following five groups: noninfected; saline-treated Plasmodium berghei-infected; LXA_{4} -pretreated P. berghei-infected (LXA_{4} administered 1 h before infection and daily, from days 0 to 5 postinfection), LXA_{4} – and LXA_{4} receptor antagonist BOC-2-pretreated P. berghei-infected; and LXA_{4} -posttreated P. berghei-infected (LXA_{4} administered from days 3 to 5 postinfection). By day 6, pretreatment or posttreatment with LXA_{4} ameliorate lung mechanic dysfunction reduced alveolar collapse, thickening and interstitial edema; impaired neutrophil accumulation in the pulmonary tissue and blood; and reduced the systemic production of CXCL1. Additionally, in vitro treatment with LXA_{4} prevented neutrophils from migrating toward plasma collected from P. berghei-infected mice. LXA_{4} also impaired neutrophil cytoskeleton remodeling by inhibiting F-actin polarization. Ex vivo analysis showed that neutrophils from pretreated and posttreated mice were unable to migrate. In conclusion, we demonstrated that LXA_{4} exerted therapeutic effects in malaria-induced ALI by inhibiting lung dysfunction, tissue injury, and neutrophil accumulation in lung as well as in peripheral blood. Furthermore, LXA_{4} impaired the migratory ability of P. berghei-infected mice neutrophils.
【저자키워드】 lung, specialized proresolving mediators, Plasmodium berghei, cytoskeleton remodeling, LXA4 receptor,