mRNA and Adenovirus vaccines for COVID-19 are used to induce humoral and cell-mediated immunity, with the aim to generate both SARS-CoV-2 B and T memory cells. In present study, we described a simple assay to detect and quantify Spike-specific CD4 + and CD8 + T cell responses induced by vaccination in healthy donors and in subjects with B cell compart impairment, in which antibody response is absent due to primary immunodeficiencies or CD20 depleting therapy. We detect and quantified memory T cell immune responses against SARS-CoV-2 evocated by vaccination in both groups, irrespective to the humoral response. Furthermore, we identified TNF-α as the main cytokine produced by T memory cells, after antigen-specific stimulation in vitro, that could be considered, other than IFN-γ, an additional biomarker of induction of T memory cells upon vaccination. Further studies on the vaccine-induced T cell responses could be crucial, not only in healthy people but also in immunocompromised subjects, where antigen specific T cells responses play a protective role against SARS-CoV-2.
【저자키워드】 SARS-CoV-2, Cytokines, CD8+, memory T cell, Pfizer/BioNTech, CD4+, 【초록키워드】 COVID-19, Vaccine, immune response, vaccination, therapy, Biomarker, Antibody Response, immunodeficiency, cytokine, in vitro, CD4, CD8, Antigen, B cell, mRNA, Immunocompromised, T cell response, Cell-mediated immunity, IFN-γ, TNF-α, CD20, humoral, T cells response, subject, protective role, impairment, memory T, T memory cells, Cell, healthy donor, produced, described, detect, healthy, generate, subjects, induce, depleting, quantified, in both groups, T memory cell, 【제목키워드】 CD4, CD8, Rapid, report, simple,