The protein array is a powerful platform to study humoral responses to infectious agents using small sample volumes [<3 μL]. Its success can be largely attributed to the development of new strategies for high-throughput cloning and expression, and improved manufacturing techniques for the construction of arrays. Here, we describe a method to hybridize protein arrays with malaria patients’ sera in order to identify seroreactive antigens, some of which may have a high potential of conferring protection from severe forms of malaria.
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【저자키워드】 protective immunity, Plasmodium falciparum, Plasmodium vivax, Malaria exposure, Seroreactive antigens, Protein arrays,
【저자키워드】 protective immunity, Plasmodium falciparum, Plasmodium vivax, Malaria exposure, Seroreactive antigens, Protein arrays,