The main objectives of developing vaccines to prevent malaria transmission are malaria control and preventing the reemergence of the disease in endemic regions. Molecular and in silico characterization of a candidate molecule is the first step in the vaccine design process. Determining the sequence and amplification of full-length cDNA copies from the mRNA transcripts is often challenging. The methods in this chapter provide a protocol for the rapid amplification of cDNA ends (RACE) and genome walking. Carboxypeptidase B2 enzyme from A. stephensi (CPBAs-2) was selected as the target molecule and the steps in its characterization and in silico analysis are explained in this chapter.
All Keywords
【저자키워드】 malaria, molecular characterization, Carboxypeptidase B2, 3′-RACE, 5′-RACE, Genome walking method, Vaccines that Interrupt Malaria Transmission (VIMTs),
【저자키워드】 malaria, molecular characterization, Carboxypeptidase B2, 3′-RACE, 5′-RACE, Genome walking method, Vaccines that Interrupt Malaria Transmission (VIMTs),