Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes acute diarrhea in suckling piglets. In Henan province of China, three swine farms broke out diarrhea in different ages of pigs during June of 2017, March of 2018 and January of 2019, respectively. PCR method, Taqman real-time RT-PCR method, sequencing, histopathology and immunohistochemistry (IHC) were conducted with the collected samples, and the results showed that PDCoV was detected among the suckling piglets, commercial fattening pigs and sows with diarrhea. PDCoV-infected suckling piglets were characterized with thin and transparent intestinal walls from colon to caecum, spot hemorrhage at mesentery and intestinal bleeding. PDCoV RNA was detected in multiple organs and tissues by Taqman real-time RT-PCR, which had high copies in ileum, inguinal lymph node, rectum and spleen. PDCoV antigen was detected in the basal layer of jejunum and ileum by IHC. In this research, we found that PDCoV could infect various ages of farmed pigs with watery diarrhea and anorexia in different seasons in a year.
【저자키워드】 qRT-PCR, diarrhea, Histopathology, PDCoV, Pig age, 【초록키워드】 immunohistochemistry, Sequencing, Novel coronavirus, Antigen, RNA, China, Research, bleeding, age, real-time RT-PCR, spleen, hemorrhage, Lymph node, acute diarrhea, Colon, tissue, anorexia, rectum, PCR method, infect, IHC, intestinal, intestinal wall, collected, conducted, characterized, cause, watery, caecum, mesentery, multiple organ, 【제목키워드】 China, age, cause,