Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of the Shigella phage Sf6, a representative member of the Podoviridae superfamily.
All Keywords
【저자키워드】 cryo-EM, bacteriophages, electron density, Localized reconstruction, Real-space refinement, Single-particle analysis,
【저자키워드】 cryo-EM, bacteriophages, electron density, Localized reconstruction, Real-space refinement, Single-particle analysis,