Background One of the most commonly applied protectotype vaccination protocol against infectious bronchitis (IB) in broiler chickens in the EU is simultaneous or alternate use of Ma5 and 4/91 vaccine strains. After IB vaccination and infection, systemic and upper respiratory tract (URT), humoral and cell-mediated immunity (CMI), are stimulated. The level of this stimulation correlates with the level of protection against IB. Results We’ve investigated the development of URT and systemic, cell-mediated and humoral immunity in commercial broiler chickens vaccinated with Ma5 and/or 4/91 strains at hatch day. We’ve demonstrated that the group vaccinated with Ma5 and 4/91 strain simultaneously developed the most desirable immunity which reflects the level of CD8 + T cells stimulation in spleen and Harderian gland, as well as the level of IgA and IgY in URT washings and serum and their cross-reactivity with 7 IBV strains. Conclusions Although we did not demonstrate directly why Ma5 + 4/91 protocol is so efficient it seems that it combines the benefits of monovalent vaccination with either Ma5 or 4/91 and while Ma5 seems to stimulate CMI more efficiently, the 4/91 strain generates a wider spectrum of immune system cross-reactivity and higher URT IgA production.
【저자키워드】 Humoral immunity, chicken, IB, Protectotype vaccination strategy, CMI, 【초록키워드】 Vaccine, vaccination, protocol, Immunity, Infection, immune system, CD8, cross-reactivity, serum, T cell, IgA, URT, Strains, upper respiratory tract, spleen, Cell-mediated immunity, strain, humoral, bronchitis, IBV, benefit, stimulated, Result, investigated, generate, applied, demonstrated, stimulate, reflect, 【제목키워드】 Efficiency, Vaccination strategy, bronchitis,