Background Porcine epidemic diarrhea virus (PEDV), a highly pathogenic and transmissible virus in swine, was first detected in the U.S. in May, 2013, and has caused tremendous losses to the swine industry. Due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated PEDV have been performed on U.S. strains in pigs. Therefore, the objective of this study was to evaluate the humoral immune response to the selected inactivated PEDV vaccine candidate in a dose-titration manner. Results PEDV was isolated from a pig with diarrhea and complete genome sequencing found >99% nucleotide identity to other U.S. PEDV. Inactivated adjuvanted monovalent vaccines were administered intramuscularly to five week old pigs in a dose titration experimental design, ranging from 6.0-8.0 log 10 tissue culture infective dose (TCID 50 /mL), to evaluate immunogenicity using a fluorescent foci neutralization assay (FFN), fluorescent microsphere immunoassay (FMIA), and enzyme-linked immunosorbent assay (ELISA) on sera. Pigs vaccinated with 8.0 log 10 TCID 50 /mL inactivated virus showed significantly higher FFN titers as well as FMIA and ELISA values than 6.0 log 10 TCID 50 /mL vaccinates and the negative controls. Conclusions These results demonstrate the immunogenicity of a PEDV inactivated viral vaccine with a U.S. strain via dose-titration. A future vaccination-challenge study would illustrate the efficacy of an inactivated vaccine and help evaluate protective FFN titers and ELISA and FMIA responses.
【저자키워드】 Vaccine, immunogenicity, diarrhea, virus, Epidemic, inactivated, PEDV, Porcine, 【초록키워드】 Efficacy, ELISA, immunoassay, Genome sequencing, Culture, Neutralization assay, Inactivated vaccine, sera, Cell culture, vaccine candidate, humoral immune response, microsphere, Protective, nucleotide, dose, strain, pig, epidemic diarrhea, identity, tissue, negative controls, help, fluorescent, TCID, foci, infective, highly pathogenic, vaccinate, Administered, experimental design, Complete, responses, FIVE, enzyme-linked immunosorbent, log, Result, selected, performed, evaluate, caused, significantly higher, intramuscularly, monovalent vaccine, 【제목키워드】 Inactivated vaccine, disease, immunogenic, demonstrated,