In many insects, the accessory gland, a secretory tissue of the male reproductive system, is essential for male fertility. Male accessory gland is the major source of proteinaceous secretions, collectively called as seminal proteins (or accessory gland proteins), which upon transfer, manipulate the physiology and behavior of mated females. Insect hormones such as ecdysteroids and juvenoids play a key role in accessory gland development and protein synthesis but little is known about underlying molecular players and their mechanism of action. Therefore, in the present study, we examined the roles of hormone-dependent transcription factors (Nuclear Receptors), in accessory gland development, function and male fertility of a genetically tractable insect model, Drosophila melanogaster . First, we carried out an RNAi screen involving 19 hormone receptors, individually and specifically, in a male reproductive tissue (accessory gland) for their requirement in Drosophila male fertility. Subsequently, by using independent RNAi/ dominant negative forms, we show that Ecdysone Receptor (EcR) is essential for male fertility due to its requirement in the normal development of accessory glands in Drosophila: EcR depleted glands fail to make seminal proteins and have dying cells. Further, our data point to a novel ecdysone receptor that does not include Ultraspiracle but is probably comprised of EcR isoforms in Drosophila male accessory glands. Our data suggest that this novel ecdysone receptor might act downstream of homeodomain transcription factor paired (prd) in the male accessory gland. Overall, the study suggests novel ecdysone receptor as an important player in the hormonal regulation of seminal protein production and insect male fertility. Author summary Insects are the major contributors to biodiversity and have economic, agricultural and health importance. This unparalleled abundance of insects, in part, can be attributed to their high reproductive potential. In many insects, proteins derived from the accessory gland, the secretory tissue of male reproductive system, are critical for fertility. The production of these accessory gland proteins is regulated by insect hormones but the underlying mechanisms/molecular players remain poorly understood. Elucidation of the same has potential applications in designing pest control management strategies and to understand the effect of environmental chemicals on reproduction. In view of this, we analyzed the role, if any, of various insect hormone receptors in development and function of the male accessory gland in a genetically tractable insect model, Drosophila melanogaster . Here, we report the involvement of Ecdysone receptor (EcR with novel composition) in Drosophila male fertility. We show that the depletion of this receptor causes cell death in male accessory glands, which fail to produce seminal fluid proteins leading to sterility/sub-fertility of Drosophila males. These findings will find potential applications in designing insect pest control strategies.
【초록키워드】 Proteins, Seminal fluid, Protein, Health, cells, management, Physiology, male, hormone, Nuclear receptors, Behavior, Fertility, reproductive system, receptors, receptor, molecular, Insects, cell death, mechanism of action, Critical, Biodiversity, protein synthesis, male fertility, transcription factor, transcription factors, Drosophila melanogaster, hormone receptors, pest control, male reproductive system, secretory tissue, (Nuclear Receptors), hormone receptor, Drosophila, Regulation, tissue, transfer, Depletion, dying cells, Ecdysone, reproduction, sterility, insect, downstream, forms, isoform, females, males, independent, analyzed, examined, carried, include, cause, regulated, reproductive, dominant negative, 【제목키워드】 male, receptor,