Labelled F-actin with FITC-phalloidin, we observed F-actin rearrangements by Streptococcus pneumoniae adhesion of type II pneumocytes (A549). Invasion of S. pneumoniae to A549 cells was determined by pretreating A549 cells with cytochalasin D. To investigate whether F-actin rearrangements can be blocked by Ca2+ inhibitors, A549 cells were pretreated with Ca2+ inhibitors datrollene. A549 cells were loaded with Fura-2/AM to determine the concentration of cytosolic free calcium by S. pneumoniae adhesion of A549 cells after 30,60,90 minutes respectively. Intact S. pneumoniae can promote F-actin rearrangements. Cytochalasin D is able to prevent S. pneumoniae invasion of A49 cells. S. pneumoniae adhesion of A549 cells increased cytosolic free calcium after 30, 60, 90 minuts. Ca2+ inhibitors datrollene block F-actin rearrangements dose dependently. It suggested S. pneumoniae can provoke F-actin rearrangements through Ca2+ signaling pathways, which will further lead to S. pneumoniae invasion of A49 cells.
[Ca2+ signaling pathways associated with the invasion of Streptococcus pneumoniae to type II pneumocytes]
[Category] 폐렴구균 감염증,
[Article Type] article
[Source] pubmed
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