We report the case of a 77-year-old woman affected by coronavirus disease-19 (COVID-19) who developed an occlusive arterial disease of the lower limb requiring a left leg amputation. We studied the mechanisms of vascular damage by SARS-CoV-2 by means of a comprehensive multi-technique in situ analysis on the diseased popliteal arterial district, including immunohistochemistry (IHC), transmission electron microscopy (TEM) and miRNA analysis. At histological analyses, we observed a lymphocytic inflammatory infiltrate, oedema and endothelialitis of adventitial vasa vasorum while the media was normal and the intima had only minor changes. The vasa vasorum expressed the ACE2 receptor and factor VIII; compared with the controls, VEGFR2 staining was reduced. TEM analyses showed endothelial injury and numerous Weibel–Palade bodies in the cytoplasm. No coronavirus particle was seen. IL-6 protein and mRNA, together with miR-155-5p and miRs-27a-5p, which can target IL-6, were significantly increased compared with that in the controls. Our case report suggests an involvement of adventitial artery microcirculation by inflammation in the course of COVID-19. Without evident signs of current infection by SARS-CoV-2, endothelial cells show a spectrum of structural and functional alterations that can fuel the cardiovascular complications observed in people infected with SARS-CoV-2.
【저자키워드】 COVID-19, SARS-CoV-2, Inflammation, immunohistochemistry, IL-6, miRNA, transmission electron microscopy, artery disease, 【초록키워드】 coronavirus, Infection, media, ACE2 receptor, Case report, Protein, Coronavirus disease-19, mRNA, endothelial injury, disease, mechanism, Endothelial cell, Analysis, Inflammatory, Vascular damage, cytoplasm, lower limb, changes, cardiovascular complication, alteration, staining, oedema, occlusive, IHC, TEM, controls, significantly increased, Course, affected, functional, expressed, analyses, was reduced, histological, infected with SARS-CoV-2, popliteal, 【제목키워드】 target, Microcirculation, Major,