A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation.
All Keywords
【저자키워드】 Salmonella Pullorum, Chicken embryo, Signature-tagged mutagenesis,
【저자키워드】 Salmonella Pullorum, Chicken embryo, Signature-tagged mutagenesis,