Background Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin. Results By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9% sensitivity (95% CI 86 to 99.5%) and 100% specificity (95% CI 78.2–100%). Conclusion The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2. Supplementary Information The online version contains supplementary material available at 10.1186/s41182-021-00396-y.
【저자키워드】 COVID-19, SARS-CoV-2, Nasopharyngeal swab, RT-LAMP, 【초록키워드】 Infection, diagnostic, RNA, amplification, sensitivity, specificity, reverse transcription, isothermal amplification, Swab, supplementary material, 95% CI, RNA purification, limit, current, Result, develop, applied, amplify, diagnosis of SARS-CoV-2, SARS-CoV-2 swab sample, Typical, 【제목키워드】 RNA, reverse transcription, isothermal amplification,