Background: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirus nucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh. Methods: Viral RNA was evaluated in swab specimens from 115 COVID-19 patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virus E-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19 patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA. Results: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9–23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27–30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsid IgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations. Conclusions: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19 patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.
【저자키워드】 COVID-19, SARS-CoV-2, IgG, IgM, RT-PCR, ELISA, Dengue fever, IgA, N-protein, DENV, 【초록키워드】 antibodies, coronavirus, pandemic, antibody, Antibody Response, Infectious disease, virus, RNA, serum, Region, Viral load, nucleocapsid, Research, Control, reverse transcription, Viral RNA, Oropharyngeal, COVID-19 patients, Coronavirus-2, Concentration, anti-nucleocapsid antibody, COVID-19 patient, COVID-19 symptom, acute respiratory syndrome, IgG levels, correlations, positive, Affect, polymerase chain, evaluated, nine, median, determine, significantly higher, dengue patients, not correlated, RNA concentration, swab specimen, 【제목키워드】 detection, Dengue,