The pandemic caused by the SARS-CoV-2 infection affects many aspects of public health knowledge, science, and practice around the world. Several studies have shown that SARS-CoV-2 RNA in plasma seems to be associated with a worse prognosis of COVID-19. In the present study, we investigated plasma and buffy RNA in patients with COVID-19 to determine its prognostic value. A prospective study was carried out in patients hospitalized for COVID-19, in which RNA was analyzed in plasma and the buffy coat. Morphological and immunohistochemical studies were used to detect the presence of SARS-CoV-2 in the buffy coat. In COVID-19 patients, the obtained RNA concentration in plasma was 448.3 ± 31.30 ng/mL. Of all the patients with positive plasma tests for SARS-CoV-2, 46.15% died from COVID-19. In four cases, tests revealed that SARS-CoV-2 was present in the buffy coat. Abnormal morphology of monocytes, lymphocytes and neutrophils was found. An immunohistochemical study showed positivity in mononuclear cells and platelets. Our results suggest that SARS-CoV-2 is present in the plasma. This facilitates viral dissemination and migration to specific organs, where SARS-CoV-2 infects target cells by binding to their receptors. In our study, the presence of plasma SARS-CoV-2 RNA was correlated with worse prognoses.
【저자키워드】 SARS-CoV-2, RNA, plasma, morphology, Blood, immunohistochemical, buffy coat, 【초록키워드】 COVID-19, public health, Monocytes, pandemic, knowledge, Prospective Study, neutrophil, Infection, Platelets, lymphocyte, Migration, SARS-CoV-2 RNA, receptors, dissemination, COVID-19 patients, binding, Concentration, target cell, mononuclear cell, Prognostic value, worse prognosis, organs, positive, infect, Affect, shown, analyzed, detect, caused, carried, died, the patient, investigated, facilitate, were used, determine, correlated, patients hospitalized, patients with COVID-19, the SARS-CoV-2, 【제목키워드】 report, Coat,