SARS-CoV-2 infection has caused a global pandemic that has severely damaged both public health and the economy. The nucleocapsid protein of SARS-CoV-2 is multifunctional and plays an important role in ribonucleocapsid formation and viral genome replication. In order to elucidate its functions, interaction partners of the SARS-CoV-2 N protein in human cells were identified via affinity purification and mass spectrometry. We identified 160 cellular proteins as interaction partners of the SARS-CoV-2 N protein in HEK293T and/or Calu-3 cells. Functional analysis revealed strong enrichment for ribosome biogenesis and RNA-associated processes, including ribonucleoprotein complex biogenesis, ribosomal large and small subunits biogenesis, RNA binding, catalysis, translation and transcription. Proteins related to virus defence responses, including MOV10, EIF2AK2, TRIM25, G3BP1, ZC3HAV1 and ZCCHC3 were also identified in the N protein interactome. This study comprehensively profiled the viral–host interactome of the SARS-CoV-2 N protein in human cells, and the findings provide the basis for further studies on the pathogenesis and antiviral strategies for this emerging infection.
【저자키워드】 SARS-CoV-2, viral–host interactome, ribonucleocapsid, 【초록키워드】 public health, Pathogenesis, translation, SARS-COV-2 infection, Transcription, Infection, virus, nucleocapsid protein, RNA, global pandemic, Protein, N protein, antiviral strategy, binding, G3BP1, cellular, Analysis, ZC3HAV1, calu-3 cells, ribonucleoprotein, complex, human cells, functions, HEK293T, viral genome replication, Affinity purification, biogenesis, ribosome biogenesis, TRIM25, human cell, MOV10, interaction partner, EIF2AK2, responses, caused, multifunctional, damaged, the N protein, small subunit, the SARS-CoV-2, ZCCHC3, 【제목키워드】 interactome,