Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) was isolated for the first time in Saudi Arabia from a patient suffering from atypical pneumonia. The Saudi Genome database was built by King Abdulaziz Medical City via the next-generation sequencing of 7000 candidates. Method: A large list of point mutations were reported in the region of the dipeptidyl peptidase 4 (DPP4) gene. The DPP4 amino acid residues correlated to MERS-CoV entry and the site of activity of DPP4 inhibitors was investigated. We retrieved the SNPs (Single-Nucleotide Polymorphism) with a variation frequency of >0.05. Results: SNP 2:162,890,175 and SNP 2:162,891,848 in the intronic region were located within 50 bp of amino acid residues responsible for MERS-CoV entry, amino acids 259–296 and 205–258, respectively. The variation frequency of SNP 2:162,890,175 was 2321 out of 2379 screened individuals. Moreover, mutation of SNP 2:162,891,848, which is located near amino acid residues E205 and E206 (crucial for the activity of DPP4 inhibitors), occurred in 76 out of 2379 screened individuals. Conclusions: Our study shows high variation frequency in the DPP4 region reported in the Saudi Genome database. The identified SNPs are of high significance for MERS-CoV infection in better understanding disease pathogenesis.
【저자키워드】 MERS-CoV, DPP4, Dipeptidyl peptidase 4, Middle East respiratory syndrome, 【초록키워드】 Mutation, Pneumonia, Variation, inhibitors, database, Saudi Arabia, Next-generation sequencing, Patient, SNP, inhibitor, Amino acid, Frequency, Point mutation, Atypical, Middle East, Candidates, disease pathogenesis, city, respiratory syndrome coronavirus, MERS-CoV infection, intronic region, amino acid residue, responsible, occurred, reported, investigated, screened, correlated, individuals, MERS-CoV entry, King, retrieved, 【제목키워드】 respiratory, Middle East, insight,