This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.
All Keywords
【저자키워드】 detection, Real-time PCR, Meningitis, Bacteria, triplex, cerebrospinal,
【저자키워드】 detection, Real-time PCR, Meningitis, Bacteria, triplex, cerebrospinal,