CFU- and confocal microscopy-based in vitro methods to assess pneumococcal adhesion and invasion of relevant human cells, such as neurons, remain a powerful tool to understand the basis of host-pathogen interactions. In recent years, there has been a continuous refinement of confocal detection of human and bacterial cells through the use of specific, fluorochrome-labelled antibodies. Used in combination, these assays provide both the means for quantification and enough flexibility to accommodate specific experimental needs.
All Keywords
【저자키워드】 immunofluorescence, Streptococcus pneumoniae, colony forming units, Adhesion assay, gentamicin protection assay, Neuronal differentiation, SH-SY5Y cell line,
【저자키워드】 immunofluorescence, Streptococcus pneumoniae, colony forming units, Adhesion assay, gentamicin protection assay, Neuronal differentiation, SH-SY5Y cell line,