The cellular response to SARS-CoV-2 vaccination and infection in allogeneic hematopoietic stem cell transplant (HSCT) recipients is not yet clear. In the current study, HSCT recipients prior to and post vaccination were tested for SARS-CoV-2-specific humoral and cellular immunity. Antibodies against spike (S) 1 were assessed by Anti-SARS-CoV-2 IgG ELISA (Euroimmun). Cellular immunity was analyzed by an in house interferon-gamma ELISpot and T-SPOT. COVID (Oxford Immunotec), using altogether seven SARS-CoV-2-specific antigens. In 117 HSCT patients vaccinated twice, SARS-CoV-2 IgG antibodies were significantly higher than in HSCT controls pre vaccination ( p < 0.0001). After the second vaccination, we observed a median antibody ratio of 4.7 and 68% positive results, whereas 35 healthy controls reached a median ratio of 9.0 and 100% positivity. ELISpot responses in patients were significantly ( p < 0.001) reduced to ≤33% of the controls. After the second vaccination, female HSCT patients and female healthy controls showed significantly higher antibody responses than males (6.0 vs. 2.1 and 9.2 vs. 8.2, respectively; p < 0.05). Cellular immunity was diminished in patients irrespective of sex. In conclusion, especially male HSCT recipients showed impaired antibody responses after SARS-CoV-2 vaccination. Changing the vaccine schedule or composition could help increase vaccine responses.
【저자키워드】 antibodies, SARS-CoV-2, vaccination, ELISPOT, allogeneic hematopoietic stem cell transplantation, sex-dependency, 【초록키워드】 Immunity, antibody, Antibody Response, Infection, Sex, COVID, cellular immunity, SARS-CoV-2 IgG antibody, response, male, female, Patient, Control, antigens, Allogeneic, IgG ELISA, SARS-CoV-2 vaccination, cellular response, humoral, vaccine responses, HSCT, second vaccination, Oxford, healthy control, help, positive, recipient, post vaccination, controls, Cell, Seven, tested, analyzed, significantly, reduced, median, significantly higher, reached, Changing, the vaccine, HSCT patient, HSCT recipient, impaired antibody response, were assessed, 【제목키워드】 Transplant, response, recipient, hematopoietic, STEM,