Background: Studies reporting the long-term humoral response after receiving the BNT162b2 COVID-19 vaccine are important to drive future vaccination strategies. Yet, available literature is scarce. Covidiagnostix is a multicenter study designed to assess the antibody response in >1000 healthcare professionals (HCPs) who received the BNT162b2 vaccine. Methods: Serum was tested at time-0 (T 0 ), before the first dose, T 1 , T 2, and T 3 , respectively, 21, 42, and 180 days after T 0 . Antibodies against the SARS-CoV-2 nucleocapsid-protein were measured to assess SARS-CoV-2 infections, whereas antibodies against the receptor-binding domain of the spike protein were measured to assess the vaccine response. Neutralization activity against the D614G, B.1.1.7, and B.1.351 variants were also analyzed. Results: Six months post-vaccination HCPs showed an antibody titer decrease of approximately 70%, yet, the titer was still one order of magnitude higher than that of seropositive individuals before vaccination. We identified 12 post-vaccination infected HCPs. None showed severe symptoms. Interestingly, most of them showed titers at T 2 above the neutralization thresholds obtained from the neutralization activity experiments. Conclusion: Vaccination induces a humoral response which is well detectable even six months post-vaccination. Vaccination prevents severe COVID-19 cases, yet post-vaccination infection is possible even in the presence of a high anti-S serum antibody titer.
【저자키워드】 COVID-19, serological test, immune response, vaccination, mRNA vaccine, Roche anti-SARS-CoV-2-S, 【초록키워드】 BNT162b2 vaccine, COVID-19 vaccine, severe COVID-19, antibody, neutralization, Infection, BNT162b2, B.1.1.7, Vaccination strategies, Antibody titer, D614G, serum antibody, multicenter, threshold, SARS-CoV-2 infections, Healthcare professional, humoral, B.1.351 variant, first dose, severe symptoms, neutralization activity, anti-S, Prevent, decrease, hcp, analyzed, detectable, receiving, the spike protein, the receptor-binding domain, magnitude, induce, the vaccine, experiments, the antibody response, were measured, seropositive individual, T 3, the SARS-CoV-2, was tested, 【제목키워드】 SARS-CoV-2, BNT162b2, Kinetics, titer, Month,