To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare linear epitope. So far, no linear epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes linear epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved linear epitope within twelve amino acids 480 CNGVEGFNCYFP 491 on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2.
【저자키워드】 SARS-CoV-2, Spike protein, RBD, rabbit monoclonal antibody, IHC, linear B-cell epitope, 【초록키워드】 immunohistochemistry, Vaccine, S protein, antibody, SARS-CoV-2 pandemic, monoclonal antibody, Infection, viral entry, Peripheral blood, death, PBMC, epitope, binding, mAb, Amino acid, mononuclear cell, tissue, domain, receptor ACE2, human cell, polyclonal, immunized, detect, caused, conserved, reported, required, linear, can be used, functional, recognize, demonstrated, mapped, rabbit antibody, single B cell, the SARS-CoV-2, 【제목키워드】 spike, rabbit, the RBD,