Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide pandemic with at least 3.8 million deaths to date. For that reason, finding an efficient vaccine for this virus quickly became a global priority. The majority of vaccines now marketed are based on the SARS-CoV-2 spike protein that has been described as the keystone for optimal immunization. In order to monitor SARS-CoV-2 spike-specific humoral responses generated by immunization or infection, we have developed a robust and reproducible enzyme-linked immunosorbent assay (ELISA) protocol. This protocol describes a method for quantitative detection of IgG antibodies against the SARS-CoV-2 spike protein using antigen-coated microtiter plates. Results showed that antibodies could be quantified between the range of 1.953 ng/mL to 500 ng/mL with limited inter- and intra-assay variability.
【저자키워드】 COVID-19, antibodies, SARS-CoV-2, ELISA, 【초록키워드】 Vaccine, coronavirus, protocol, antibody, Infection, virus, immunization, Spike protein, IgG antibody, Humoral response, death, Quantitative, acute respiratory syndrome, worldwide pandemic, Variability, MONITOR, enzyme-linked immunosorbent, robust, Result, described, caused, majority, quantified, inter- and intra-assay, SARS-CoV-2 spike-specific, the SARS-CoV-2, 【제목키워드】 IgG, spike, Human, detection, Specific, the SARS-CoV-2,