Twenty individuals (17 females, 3 males, aged 31–65 years (range), median: 46) who received both doses of the BioNTech Pfizer mRNA vaccine were examined (11 to 31 days, median: 25) after the second dose for the presence of antibodies against peptides of SARS-COV-2 and some of MERS-CoV, SARS-CoV1, HCov229E, and HCoVNL63. Clinical evaluation revealed that six people had COVID-19 in the past. We found that: (i) Six people claimed the presence of unwanted effects of vaccination, which were more frequent in those with a history of COVID-19 (4 out of 6 vs. 2 out of 14, p = 0.037); (ii) All individuals independent of the past history of COVID-19 responded equally well in IgG but those who experienced the disease tended to do better in IgA class (729.04 vs. 529.78 U/mL, p = 0.079); (iii) All those who had experienced the disease had IgG antibodies against nucleocapsid antigens but also 5 out of 14 who had not had the disease (6/6 vs. 5/14, p = 0.014); (iv) Anti S2 antibodies were present in the patients having COVID-19 in the past but also were found in those who had not had the disease (6/6 vs. 8/14, p = 0.144); (v) All vaccinated people were highly positive in the IGRA and the level of released IFN gamma was correlated with the numbers of HLADR positive lymphocytes in the blood ( R = 0.5766, p = 0.008).
【저자키워드】 COVID-19, SARS-CoV-2, mRNA vaccine, antibodies response, IFN gamma blood cells release, 【초록키워드】 IgG, vaccination, antibody, peptide, MERS-CoV, Pfizer, lymphocyte, IgG antibody, clinical, IFN, Blood, nucleocapsid antigen, dose, second dose, individual, positive, HLADR, IgA class, Effect, females, males, independent, examined, the patient, the disease, correlated, released, presence of antibody, claimed, 【제목키워드】 mRNA, response,