Coronavirus disease (COVID-19) causes a serious threat to human health. Virus-like particles (VLPs) constitute a promising platform in SARS-CoV-2 vaccine development. In this study, the E, M, and S genes were cloned into multiple cloning sites of a new triple expression plasmid with one p10 promoter, two pPH promoters, and three multiple cloning sites. The plasmid was transformed into DH10 Bac TM Escherichia coli competent cells to obtain recombinant bacmid. Then the recombinant bacmid was transfected in ExpiSf9 TM insect cells to generate recombinant baculovirus. After ExpiSf9 TM cells infection with the recombinant baculovirus, the E, M, and S proteins were expressed in insect cells. Finally, SARS-CoV-2 VLPs were self-assembled in insect cells after infection. The morphology and the size of SARS-CoV-2 VLPs are similar to the native virions.
【저자키워드】 SARS-CoV-2, virus-like particles, insect cells, 【초록키워드】 COVID-19, S protein, Infection, SARS-CoV-2 vaccine, Particle, Health, morphology, disease, expression, platform, baculovirus, S gene, Plasmid, Escherichia coli, promoters, virions, promoter, VLPs, Cell, cloned, generate, cause, insect cell, were expressed, competent, bacmid, multiple cloning site, SARS-CoV-2 VLP, 【제목키워드】 Production, Particle, insect,