Background Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution. Results SARS-CoV detection rates in sera were highest in the first 9 days of illness, whereas detection was highest in throat washes 5–14 days after onset of symptoms. The highest SARS-CoV RT-PCR rates (70.4–86.3%) and viral loads (log 10 4.5–6.1) were seen in fecal samples collected 2–4 weeks after the onset of clinical illness. Fecal samples were frequently SARS-CoV RT-PCR positive beyond 40 days, and occasional sera still had SARS-CoV detected after 3 weeks of illness. Conclusion In the context of an extensive outbreak with major pressure on hospital resources, patient self-collected samples are an alternative to nasopharyngeal aspirates for laboratory confirmation of SARS-CoV infection.
【초록키워드】 coronavirus, Pneumonia, SARS-CoV, severe, RT-PCR, China, Viral load, outbreak, sera, Patient, Fecal sample, detection rate, reverse transcriptase, acute respiratory syndrome, nasopharyngeal aspirate, Beijing, SARS-CoV infection, positive, laboratory confirmation, laboratory-confirmed, hospital resources, polymerase chain, log, detected, clinical sample, Result, highest, 2003, was used, detect, collected, caused, onset of symptoms,