The emergence and rapid spread of the B.1.1.7 lineage (VOC-202012/01) SARS-CoV-2 variant has aroused global concern. The N501Y substitution is the only mutation in the interface between the RBD of B.1.1.7 and ACE2, raising concerns that its recognition by neutralizing antibodies may be affected. Here, we assessed the neutralizing activity and binding affinity of a panel of 12 monoclonal antibodies against the wild type and N501Y mutant SARS-CoV-2 pseudovirus and RBD protein, respectively. We found that the neutralization activity and binding affinity of most detected antibodies (10 out of 12) were unaffected, although the N501Y substitution decreased the neutralizing and binding activities of CB6 and increased that of BD-23. These findings could be of value in the development of therapeutic antibodies. Supplementary Information The online version contains supplementary material available at 10.1186/s12985-021-01554-8.
【저자키워드】 SARS-CoV-2, Neutralizing activity, N501Y variant, binding kinetics, Monoclonal neutralizing antibody, 【초록키워드】 neutralizing antibody, antibodies, ACE2, Mutation, monoclonal antibody, SARS-CoV-2 variant, binding affinity, B.1.1.7 lineage, Spread, pseudovirus, B.1.1.7, N501Y, therapeutic, VOC-202012/01, Neutralizing, wild type, RBD protein, binding activity, supplementary material, neutralization activity, Mutant SARS-CoV-2, affected, detected antibody, the RBD, raising, 【제목키워드】 spike, N501Y, Impact, epitope, neutralizing monoclonal antibody,