Background Porcine epidemic diarrhea virus (PEDV) is an important pathogen in swine and is responsible for substantial economic losses. Previous studies suggest that the PEDV E protein plays an important role in the viral assembly process. However, the subcellular localization and other functions of PEDV E protein still require more research. Methods The subcellular localization and function of PEDV E protein were investigated by examining its effects on cell growth, cell cycle progression, interleukin-8 (IL-8) expression and cell survival. Results The results show that plenty of PEDV E protein is localized in the ER, with small quantities localized in the nucleus. The PEDV E protein has no effect on the intestinal epithelial cells (IEC) growth, cell cycle and cyclin A expression. The cells expressing PEDV E protein express higher levels of IL-8 than control cells. Further studies show that PEDV E protein induced endoplasmic reticulum (ER) stress and activated NF-κB which is responsible for the up-regulation of IL-8 and Bcl-2 expression. Conclusions This study shows that the PEDV E protein is localized in the ER and the nucleus and it can cause ER stress. The PEDV E protein had no effect on the IEC growth and cell cycle. In addition, the PEDV E protein is able to up-regulate IL-8 and Bcl-2 expression.
【저자키워드】 E protein, IL-8, ER stress, PEDV, BCL-2, NF-Κb, 【초록키워드】 Stress, virus, Endoplasmic reticulum, E protein, survival, pathogen, cells, Research, IL-8, expression, ER stress, function, NF-κB, the cell, epidemic diarrhea, growth, PEDV, previous study, Express, nucleus, BCL-2, subcellular localization, Effect, Cell, Result, responsible, addition, investigated, activated, cause, had no, expressing, up-regulate, cell cycle progression, IEC, intestinal epithelial cell,