Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.
【초록키워드】 Human, Infection, virus, Protein, Gene, Real-time PCR, viral replication, cytomegalovirus, experiment, yellow fever, SARS Coronavirus, expression, binding, cellular, Analysis, criteria, β-actin, expression level, infected cells, ranking, cell line, quantitative real-time PCR, mRNA expression, Infected, reference, reference gene, compared, tested, determined, were infected, reported, Camelpox,