Background A serological method to simultaneously detect antibodies against infectious laryngotracheitis virus (ILTV) and infectious bronchitis virus (IBV) is imperative for the differential diagnosis and evaluation of antibodies titers after vaccination. Method The microspheres coated with purified recombinant glycoprotein D (gD) of ILTV or nucleocapsid (N) protein of IBV were incubated with serum samples. The simultaneous quantification of ILTV and IBV antibodies were achieved through the interrogation of microspheres by Luminex 200 detection system. . Results This xMAP detection demonstrated no nonspecific reactions with avian influenza virus (AIV), avian leukosis virus (ALV), newcastle disease virus (NDV), and Marek’s disease virus (MDV). The results also demonstrated that the xMAP assay was four times more sensitive than the enzyme-linked immunosorbent assay (ELISA) for ILTV detection and two times more sensitive for IBV detection. A total of 90 chicken serum samples from a chicken farm were tested by xMAP and ELISA assays. The results showed that the coincidence rates were 84.44 and 100% for ILTV and IBV detection, respectively. Conclusion This study exhibited an opportunity for the differential diagnosis through simultaneous detection of multiplex antibodies in serum and can be used for the multiplex antibodies evaluation after vaccination.
【저자키워드】 Luminex xMAP technology, Infectious laryngotracheitis virus, Infectious bronchitis virus, antibody detection, ELISA, 【초록키워드】 vaccination, antibody, Influenza virus, virus, ELISA, Protein, serum, nucleocapsid, glycoprotein, multiplex, serological, quantification, disease, differential diagnosis, microsphere, serum samples, reaction, Infectious Bronchitis Virus, ELISA assays, serum sample, IBV, enzyme-linked immunosorbent, detection system, Result, tested, detect, exhibited, can be used, demonstrated, imperative, purified, incubated, coated with, nonspecific, 【제목키워드】 antibody, virus, development, bronchitis,