Cross-reactive T cell immunity to seasonal coronaviruses (HCoVs) may lead to immunopathology or protection during SARS-CoV2 infection. To understand the influence of cross-reactive T cell responses, we used IEDB (Immune epitope database) and NetMHCpan (ver. 4.1) to identify candidate CD8 + T cell epitopes, restricted through HLA-A and B alleles. Conservation analysis was carried out for these epitopes with HCoVs, OC43, HKU1, and NL63. 12/18 the candidate CD8 + T cell epitopes (binding score of ≥0.90), which had a high degree of homology (>75%) with the other three HCoVs were within the NSP12 and NSP13 proteins. They were predicted to be restricted through HLA-A*2402, HLA-A*201, HLA-A*206, and HLA-B alleles B*3501. Thirty-one candidate CD8 + T cell epitopes that were specific to SARS-CoV2 virus (<25% homology with other HCoVs) were predominantly identified within the structural proteins (spike, envelop, membrane, and nucleocapsid) and the NSP1, NSP2, and NSP3. They were predominantly restricted through HLA-B*3501 (6/31), HLA-B*4001 (6/31), HLA-B*4403 (7/31), and HLA-A*2402 (8/31). It would be crucial to understand T cell responses that associate with protection, and the differences in the functionality and phenotype of epitope specific T cell responses, presented through different HLA alleles common in different geographical groups, to understand disease pathogenesis.
【저자키워드】 SARS-CoV2, seasonal coronaviruses, HLA alleles, CD8+ T cell epitopes, OC-43, HKU-1, NL-63, 【초록키워드】 Immunity, alleles, Proteins, immunopathology, virus, CD8, database, SARS-CoV2 infection, T cell, nucleocapsid, T cell responses, nsp1, phenotype, membrane, NL63, structural protein, seasonal coronavirus, conservation, epitope, T cell epitope, T cell response, OC43, HKU1, binding, Analysis, cross-reactive, T cell epitopes, HLA-A, disease pathogenesis, IEDB, NetMHCpan, homology, HLA allele, predicted, identify, carried, groups, HLA-B allele, 【제목키워드】 CD8, novel, Other, identification, candidate, seasonal,