SARS-CoV-2 has spread across the globe with an astonishing velocity and lethality that has put scientist and pharmaceutical companies worldwide on the spot to develop novel treatment options and reliable vaccination for billions of people. To combat its associated disease COVID-19 and potentially newly emerging coronaviruses, numerous pre-clinical cell culture techniques have progressively been used, which allow the study of SARS-CoV-2 pathogenesis, basic replication mechanisms, and drug efficiency in the most authentic context. Hence, this review was designed to summarize and discuss currently used in vitro and ex vivo cell culture systems and will illustrate how these systems will help us to face the challenges imposed by the current SARS-CoV-2 pandemic.
【저자키워드】 SARS-CoV-2, Cell culture, human lung organoids, in vitro lung model, human airway epithelial cell culture, lung-on-a-chip, ex vivo lung, air–liquid interface, 【초록키워드】 COVID-19, Treatment, Coronaviruses, vaccination, SARS-CoV-2 pandemic, in vitro, Replication, Spread, mechanisms, disease, SARS-CoV-2 pathogenesis, Efficiency, Ex vivo, help, develop, globe, cell culture system, 【제목키워드】 Model, application,