Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
【저자키워드】 COVID-19, SARS-CoV-2, coronavirus, lysis buffer, inactivation, extraction buffer, 【초록키워드】 coronavirus disease, protocol, diagnostic, risk, Laboratory, biosafety, RNA extraction, clinical, Patient, molecular, COVID-19 patients, Infectious virus, Contact, reduction, French, supernatant, nasopharyngeal sample, European, effective, log, clinical sample, tested, collected, evaluated, were used, less, reduce, 【제목키워드】 Chemical, Sample,