Human coronavirus OC43 (HCoV-OC43) is one of the coronaviruses causing a mild common cold, but few studies have been made on this strain. Here, we identified the molecular mechanisms involved in HCoV-OC43-induced apoptosis and its implications for viral reproduction in Vero cells and MRC-5 cells. HCoV-OC43 infection induced apoptosis that was accompanied by cleavage of caspase-3 and PARP, degradation of cyclin D1, and cell cycle arrest at S and G2M phases. Dephosphorylation of STAT1 and STAT3, induced by HCoV-OC43 infection, was also associated with HCoV-OC43-mediated apoptosis. The pan-caspase inhibitor effectively prevented HCoV-OC43-induced apoptosis and reduced viral replication, suggesting that apoptosis contributes to viral replication. Collectively our results indicate that HCoV-OC43 induces caspase-dependent apoptosis to promote viral replication in Vero cells and MRC-5 cells.
【저자키워드】 Apoptosis, HCoV-OC43, Replication, 【초록키워드】 coronavirus, Human, Infection, molecular mechanism, cells, viral replication, cleavage, PARP, Degradation, Stat1, inhibitor, OC43, caspase-3, STAT3, mild common cold, dephosphorylation, cell cycle arrest, Vero Cell, caspase-dependent apoptosis, implication, involved, reduced, coronavirus, contribute, promote, induce, prevented, accompanied, cyclin D1, 【제목키워드】 Human coronavirus, OC43,