Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive and respiratory syndrome virus (PRRSV) but respond to infection via cytokine production, indicating a possible role in initiation/regulation of the immune response against PRRSV. In this work, we evaluated the responses of splenic and blood cDCs, with DEC205 + CADM1 + CD172a +/− phenotype, as well as those of CD163 + cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both populations were incubated in the presence of PRRSV or PEDV with and without naïve CD3 + T cells, and cytokine responses were evaluated by qPCR and ELISA. Our results showed that cDCs, but not CD163 + cells, produced IL-12 in response to PRRSV. PEDV did not induce IL-12 production. Cocultures of cDCs and autologous naïve CD3 + cells resulted in decreased IL-12 production and low expression of IFN-γ transcripts in response to PRRSV. Interestingly, cDCs increased the proliferation of naïve T cells in the presence of PRRSV compared with that achieved with monocytes and peripheral blood mononuclear cells (PBMCs). Cocultures of CD163 + cells induced IL-10 and IL-4 expression in the presence of PRRSV and PEDV, respectively. In conclusion, cDCs can selectively produce IL-12 in response to PRRSV but poorly participate in the activation of naïve T cells.
【저자키워드】 dendritic cells, CDC, PEDV, IL-12, PRRSV, DEC205, naïve T cells, 【초록키워드】 immune response, T cells, Infection, virus, ELISA, Population, monocyte, Peripheral blood, cells, qPCR, response, phenotype, IL-10, expression, dendritic cell, cytokine production, Blood, IFN-γ, IL-4, PBMCs, cytokine response, proliferation, mononuclear cell, epidemic diarrhea, Activation, low expression, syndrome, naïve, CD163, naïve T cells, transcript, CD3, Cell, autologous, coculture, produced, evaluated, induce, respond, reproductive, incubated, CADM1, Conventional, naïve T cell, 【제목키워드】 response, CADM1, Conventional,