Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, is the causative agent of porcine epidemic diarrhea (PED) that damages intestinal epithelial cells and results in severe diarrhea and dehydration in neonatal suckling pigs with up to 100% mortality. The oral vaccine route is reported as a promising approach for inducing protective immunity against PEDV invasion. Furthermore, dendritic cells (DCs), professional antigen-presenting cells, link humoral and cellular immune responses for homeostasis of the intestinal immune environment. In this study, in order to explore an efficient oral vaccine against PEDV infection, a mucosal DC-targeting oral vaccine was developed using Lactobacillus casei to deliver the DC-targeting peptide (DCpep) fused with the PEDV core neutralizing epitope (COE) antigen. This probiotic vaccine could efficiently elicit secretory immunoglobulin A (SIgA)-based mucosal and immunoglobulin G (IgG)-based humoral immune responses via oral vaccination in vivo. Significant differences ( p < 0.05) in the immune response levels were observed between probiotics expressing the COE-DCpep fusion protein and COE antigen alone, suggesting better immune efficiency of the probiotics vaccine expressing the DC-targeting peptide fused with PEDV COE antigen. This mucosal DC-targeting oral vaccine delivery effectively enhances vaccine antigen delivery efficiency, providing a useful strategy to induce efficient immune responses against PEDV infection.
【저자키워드】 Oral vaccine, PEDV COE antigen, dendritic cell-targeting peptide, lactobacillus, 【초록키워드】 Vaccine, coronavirus, immune response, vaccination, Mortality, Cellular immune response, Infection, peptide, diarrhea, virus, immune, Antigen, Immunoglobulin, protective immunity, Neutralizing, humoral immune response, fusion protein, in vivo, epitope, homeostasis, dendritic cell, mucosal, Invasion, Efficiency, Neonatal, secretory immunoglobulin A, humoral, epidemic diarrhea, antigen-presenting cells, PEDV, vaccine antigen, DCs, approach, intestinal, ENhance, reported, elicit, induce, expressing, Significant, intestinal epithelial cell, fused, COE, Lactobacillus casei, 【제목키워드】 Strategy, Probiotics, delivery, oral, PEDV, COE,