Porcine Epidemic Diarrhea Virus (PEDV) is the causative agent of swine epidemic diarrhea. In order to study the pathogenic mechanism of PEDV, PEDV was inoculated into Vero cells cultured in vitro, and the total RNA of Vero cells was extracted to construct a library for Illumina high-throughput sequencing and screening of differentially expressed genes ( p < 0.05). Five differentially expressed genes for qRT-PCR verification analysis were randomly selected, and the verification results were consistent with the transcriptome sequencing results. The Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway enrichment analysis was performed on the differentially expressed genes screened above. The results showed that the target gene annotations of differentially expressed genes in the African green monkey genome were mainly enriched in the TNF signaling pathway, the P53 signaling pathway, the Jak-STAT signaling pathway, the MAPK signaling pathway, and immune inflammation. In addition, it has been reported that Puma can promote apoptosis and is a key mediator of P53-dependent and non-dependent apoptosis pathways. However, there is no report that PEDV infection can activate Puma and induce apoptosis in a P53-dependent pathway. It was found by flow cytometry that PEDV infection induced apoptosis, and by Western Blotting detection, PEDV infection significantly increased the expression of p53, BAX, and Puma apoptosis-related proteins. Treatment Vero cells with the p53 inhibitor, PFT-α, could significantly inhibit PEDV-induced apoptosis. Studies have shown that PEDV infection can activate Puma and induce apoptosis in a P53-dependent pathway. These findings provide data support for further elucidating the pathogenic mechanism of PEDV and developing an effective vaccine against PEDV.
【저자키워드】 Apoptosis, transcriptome sequencing, Porcine epidemic diarrhea virus, puma, p53 signaling pathway, BAX/Bcl-2, 【초록키워드】 Inflammation, Vaccine, qRT-PCR, Sequencing, Genome, Infection, Proteins, in vitro, flow cytometry, immune, pathway, signaling pathway, inhibitor, African green monkey, Illumina, expression, mechanism, differentially expressed gene, p53, Analysis, Pathway enrichment analysis, target gene, epidemic diarrhea, Support, PEDV, pathogenic, annotation, KEGG, MAPK signaling, total RNA, apoptosis pathways, Vero Cell, effective, Randomly, TNF signaling, Kyoto, significantly increased, selected, shown, significantly, reported, inoculated, addition, inhibit, was performed, screened, promote, induce, activate, Blotting, 【제목키워드】 Vero,