The QX-type avian infectious bronchitis virus (IBV) is still a prevalent genotype in Southwestern China. To analyze the antigenicity and pathogenicity characteristics of the dominant genotype strains (QX-type), S1 gene sequence analysis, virus cross-neutralization tests, and pathogenicity test of eight QX-type IBV isolates were conducted. Sequence analysis showed that the nucleotide homology between the eight strains was high, but distantly related to H120 and 4/91 vaccine strains. Cross-neutralization tests showed that all eight strains isolated from 2015 and 2017 belonged to the same serotype, but exhibited antigenic variations over time. The pathogenicity test of the five QX-type IBV isolates showed that only three strains, CK/CH/SC/DYW/16, CK/CH/SC/MS/17, and CK/CH/SC/GH/15, had a high mortality rate with strong respiratory and renal pathogenicity, whereas CK/CH/SC/PZ/17 and CK/CH/SC/DYYJ/17 caused only mild clinical symptoms and tissue lesions. Our results indicate that the prevalent QX-type IBVs displayed antigenic variations and pathogenicity difference. These findings may provide reference for research on the evolution of IBV and vaccine preparation of infectious bronchitis (IB).
【저자키워드】 antigenic, Infectious Bronchitis Virus, pathogenic, IBV, QX, 【초록키워드】 Evolution, Vaccine, virus, China, Characteristics, cross-neutralization, Research, Genotype, antigenicity, pathogenicity, Strains, nucleotide, Analysis, antigenic variation, strain, serotype, bronchitis, homology, renal, high mortality rate, dominant, isolate, FIVE, prevalent, tissue lesions, gene sequence, caused, conducted, eight, exhibited, mild clinical symptom, 【제목키워드】 China, strain, Isolated,