Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.
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【저자키워드】 SARS-CoV-2, coronavirus, host factors, Anti-viral, flavivirus, Genome editing, viral life cycle, CRISPR KO, CRISPRa, pro-viral, 【초록키워드】 viruses, knowledge, COVID-19 pandemic, Genome, diagnostics, CRISPR, viral replication, understanding, disease, Critical, mechanism, cellular, Activation, Factor, viral pathogen, screening method, Host, robust, 【제목키워드】 CRISPR,
【저자키워드】 SARS-CoV-2, coronavirus, host factors, Anti-viral, flavivirus, Genome editing, viral life cycle, CRISPR KO, CRISPRa, pro-viral, 【초록키워드】 viruses, knowledge, COVID-19 pandemic, Genome, diagnostics, CRISPR, viral replication, understanding, disease, Critical, mechanism, cellular, Activation, Factor, viral pathogen, screening method, Host, robust, 【제목키워드】 CRISPR,