The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells. Co-immunoprecipitation (CO-IP) results clearly demonstrated that PEDV N protein could bind to human hnRNP A1. Replication of PEDV was inhibited by silencing the expression of hnRNP A1 in CCL-81 cells, suggesting the positive effect of hnRNP A1 on PEDV infection.
【저자키워드】 nucleocapsid protein, porcine epidemic diarrhea virus (PEDV), heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), 【초록키워드】 translation, Infection, virus, Replication, Protein, cells, nucleocapsid, N protein, in vivo, expression, cellular, ribonucleoprotein, cytoplasm, epidemic diarrhea, Regulation, infected cells, PEDV, nucleus, positive, multifunctional protein, nuclear, proteomic study, thought, predicted, inhibited, in viral, demonstrated, pre-mRNA,