In 2007, a novel coronavirus associated with an acute respiratory disease in alpacas (Alpaca Coronavirus, ACoV) was isolated. Full-length genomic sequencing of the ACoV demonstrated the genome to be consistent with other Alphacoronaviruses. A putative additional open-reading frame was identified between the nucleocapsid gene and 3’UTR. The ACoV was genetically most similar to the common human coronavirus (HCoV) 229E with 92.2% nucleotide identity over the entire genome. A comparison of spike gene sequences from ACoV and from HCoV-229E isolates recovered over a span of five decades showed the ACoV to be most similar to viruses isolated in the 1960’s to early 1980’s. The true origin of the ACoV is unknown, however a common ancestor between the ACoV and HCoV-229E appears to have existed prior to the 1960’s, suggesting virus transmission, either as a zoonosis or anthroponosis, has occurred between alpacas and humans.
【저자키워드】 coronavirus, Human, molecular evolution, reverse zoonosis, respiratory, alpaca, anthroponosis, 【초록키워드】 coronavirus, Genome, virus transmission, Novel coronavirus, Human coronavirus, humans, zoonosis, HCoV-229E, novel, respiratory, UTR, alpaca, genomic sequencing, 229E, Nucleocapsid gene, nucleotide, acute respiratory disease, identification, characterization, identity, Frame, MOST, isolate, FIVE, occurred, virus, appear, demonstrated, spike gene sequence,