SARS-CoV-2 spike protein, elaborated Vaccine development for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is focused on the trimeric spike protein that initiates infection. Each protomer in the trimeric spike has 22 glycosylation sites. How these sites are glycosylated may affect which cells the virus can infect and could shield some epitopes from antibody neutralization. Watanabe et al. expressed and purified recombinant glycosylated spike trimers, proteolysed them to yield glycopeptides containing a single glycan, and determined the composition of the glycan sites by mass spectrometry. The analysis provides a benchmark that can be used to measure antigen quality as vaccines and antibody tests are developed. Science this issue p. 330 A mass spectrometry analysis reveals the glycan composition at all glycosylation sites on the SARS-CoV-2 spike protein. The emergence of the betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), represents a considerable threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. The SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design.
【초록키워드】 COVID-19, coronavirus disease, Structure, SARS-CoV-2, Vaccine, coronavirus, glycosylation, Vaccine design, Infection, virus, Betacoronavirus, Spike protein, Antigen, Protein, immune evasion, Health, SARS-CoV-2 spike protein, Trimeric spike protein, membrane fusion, glycoprotein, humoral immune response, Antibody test, epitope, Antibody neutralization, glycan, Analysis, Science, acute respiratory syndrome, cell entry, viral spike, SARS-CoV-2 S, trimeric spike, ENCODE, spike trimers, SARS-CoV-2 S gene, infect, Host, Affect, implication, approach, Cell, trimeric, glycosylation site, can be used, provide, in viral, expressed, reveal, purified, glycosylated, initiate, the SARS-CoV-2, 【제목키워드】 Analysis, the SARS-CoV-2,