Background To contain the pandemics caused by SARS-CoV-2, early detection approaches with high accuracy and accessibility are critical. Generating an antigen-capture based detection system would be an ideal strategy complementing the current methods based on nucleic acids and antibody detection. The spike protein is found on the outside of virus particles and appropriate for antigen detection. Methods In this study, we utilized bioinformatics approaches to explore the immunodominant fragments on spike protein of SARS-CoV-2. Results The S1 subunit of spike protein was identified with higher sequence specificity. Three immunodominant fragments, Spike 56-94 , Spike 199-264 , and Spike 577-612 , located at the S1 subunit were finally selected via bioinformatics analysis. The glycosylation sites and high-frequency mutation sites on spike protein were circumvented in the antigen design. All the identified fragments present qualified antigenicity, hydrophilicity, and surface accessibility. A recombinant antigen with a length of 194 amino acids (aa) consisting of the selected immunodominant fragments as well as a universal Th epitope was finally constructed. Conclusion The recombinant peptide encoded by the construct contains multiple immunodominant epitopes, which is expected to stimulate a strong immune response in mice and generate qualified antibodies for SARS-CoV-2 detection.
【저자키워드】 COVID-19, SARS-CoV-2, Spike protein, Antigen-capture, Immunodominant fragments, 【초록키워드】 Mutation, spike, antibody, bioinformatics, peptide, Antigen, nucleic acid, SARS-CoV-2 detection, specificity, Antibody detection, mice, Pandemics, antigenicity, epitope, antigen detection, Bioinformatics analysis, Critical, immunodominant epitopes, Amino acid, S1 subunit, sequence, high accuracy, strong immune response, immunodominant, hydrophilicity, detection system, glycosylation site, Result, selected, the S1 subunit, caused, approach, generate, virus particle, expected, stimulate, 【제목키워드】 coronavirus, early diagnosis, Region, Bioinformatic, acute respiratory syndrome, immunodominant,