Many recent disease outbreaks in humans had a zoonotic virus etiology. Bats in particular have been recognized as reservoirs to a large variety of viruses with the potential to cross-species transmission. In order to assess the risk of bats in Switzerland for such transmissions, we determined the virome of tissue and fecal samples of 14 native and 4 migrating bat species. In total, sequences belonging to 39 different virus families, 16 of which are known to infect vertebrates, were detected. Contigs of coronaviruses, adenoviruses, hepeviruses, rotaviruses A and H, and parvoviruses with potential zoonotic risk were characterized in more detail. Most interestingly, in a ground stool sample of a Vespertilio murinus colony an almost complete genome of a Middle East respiratory syndrome-related coronavirus (MERS-CoV) was detected by Next generation sequencing and confirmed by PCR. In conclusion, bats in Switzerland naturally harbour many different viruses. Metagenomic analyses of non-invasive samples like ground stool may support effective surveillance and early detection of viral zoonoses.
【초록키워드】 viruses, zoonoses, Coronaviruses, coronavirus, Human, Sequencing, Genome, risk, virus, MERS-CoV, Stool, PCR, Surveillance, outbreak, zoonotic, Fecal sample, bat, etiology, Switzerland, disease, metagenomic analysis, Cross-species transmission, rotavirus, Middle East, Support, Non-invasive, tissue, sequence, transmissions, reservoir, vertebrates, MOST, infect, Complete, effective, characterized, variety, contig, Vespertilio, 【제목키워드】 virus, zoonotic, Switzerland, Endemic, Analysis, fecal, tissue sample,